Modification of Medium Composition for Enhancing the Production of Antifungal Activity from Xenorhabdus stockiae PB09 by Using Response Surface Methodology.
Identifieur interne : 000720 ( Main/Exploration ); précédent : 000719; suivant : 000721Modification of Medium Composition for Enhancing the Production of Antifungal Activity from Xenorhabdus stockiae PB09 by Using Response Surface Methodology.
Auteurs : Chirayu Sa-Uth [Thaïlande] ; Paweena Rattanasena [Thaïlande] ; Angsumarn Chandrapatya [Thaïlande] ; Prapassorn Bussaman [Thaïlande]Source :
- International journal of microbiology [ 1687-918X ] ; 2018.
Abstract
Xenorhabdus stockiae PB09 bacterium has been shown to exhibit antifungal activity against several plant pathogens. To improve its efficacy, the optimization of the nutritional components in culture media was performed. The medium components that have significant effects on antifungal activity of X. stockiae PB09 were initially identified using a fractional factorial design. Response surface methodology and central composite design were then used to create a model for optimizing the levels of carbon, nitrogen, and mineral sources that maximize antifungal activity of X. stockiae PB09. After that, the suitable carbon, nitrogen, and mineral sources were selected and adjusted by the second-order polynomial regression model, which predicted that 98.62% of antifungal activity could be obtained when the medium contained sucrose, yeast extract, NaCl, and K2HPO4 at 3.24, 23.71, 5.46, and 2.73 g/L, respectively. Laboratory verification of this recipe resulted in the antifungal activity at 97.95% in the shake flask experiment after 48-hour cultivation, which was significantly 27.22% higher than that obtained by using the TSB medium. In addition, X. stockiae PB09 cultured in the verified recipe by using 5 L fermenter could effectively inhibit the mycelial growth of Phytophthora sp., Rhizoctonia solani, Pythium sp., and Fusarium oxysporum. This study demonstrated that the RSM and CCD were shown to be valuable tools for optimizing the culture medium that maximize the antifungal activity of X. stockiae PB09.
DOI: 10.1155/2018/3965851
PubMed: 30008748
PubMed Central: PMC6020484
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Modification of Medium Composition for Enhancing the Production of Antifungal Activity from <i>Xenorhabdus stockiae</i> PB09 by Using Response Surface Methodology.</title><author><name sortKey="Sa Uth, Chirayu" sort="Sa Uth, Chirayu" uniqKey="Sa Uth C" first="Chirayu" last="Sa-Uth">Chirayu Sa-Uth</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, Faculty of Technology, Mahasarakham University, Maha Sarakham 44150, Thailand.</nlm:affiliation><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Biotechnology, Faculty of Technology, Mahasarakham University, Maha Sarakham 44150</wicri:regionArea><wicri:noRegion>Maha Sarakham 44150</wicri:noRegion></affiliation></author><author><name sortKey="Rattanasena, Paweena" sort="Rattanasena, Paweena" uniqKey="Rattanasena P" first="Paweena" last="Rattanasena">Paweena Rattanasena</name><affiliation wicri:level="1"><nlm:affiliation>Community Public Health Sub-Department, Department of Applied Sciences, Faculty of Science and Technology, Phranakhon Si Ayutthaya Rajabhat University, Ayutthaya 13000, Thailand.</nlm:affiliation><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Community Public Health Sub-Department, Department of Applied Sciences, Faculty of Science and Technology, Phranakhon Si Ayutthaya Rajabhat University, Ayutthaya 13000</wicri:regionArea><wicri:noRegion>Ayutthaya 13000</wicri:noRegion></affiliation></author><author><name sortKey="Chandrapatya, Angsumarn" sort="Chandrapatya, Angsumarn" uniqKey="Chandrapatya A" first="Angsumarn" last="Chandrapatya">Angsumarn Chandrapatya</name><affiliation wicri:level="1"><nlm:affiliation>Department of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.</nlm:affiliation><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok 10900</wicri:regionArea><wicri:noRegion>Bangkok 10900</wicri:noRegion></affiliation></author><author><name sortKey="Bussaman, Prapassorn" sort="Bussaman, Prapassorn" uniqKey="Bussaman P" first="Prapassorn" last="Bussaman">Prapassorn Bussaman</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, Faculty of Technology, Mahasarakham University, Maha Sarakham 44150, Thailand.</nlm:affiliation><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Biotechnology, Faculty of Technology, Mahasarakham University, Maha Sarakham 44150</wicri:regionArea><wicri:noRegion>Maha Sarakham 44150</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:30008748</idno><idno type="pmid">30008748</idno><idno type="doi">10.1155/2018/3965851</idno><idno type="pmc">PMC6020484</idno><idno type="wicri:Area/Main/Corpus">000716</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000716</idno><idno type="wicri:Area/Main/Curation">000716</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000716</idno><idno type="wicri:Area/Main/Exploration">000716</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Modification of Medium Composition for Enhancing the Production of Antifungal Activity from <i>Xenorhabdus stockiae</i> PB09 by Using Response Surface Methodology.</title><author><name sortKey="Sa Uth, Chirayu" sort="Sa Uth, Chirayu" uniqKey="Sa Uth C" first="Chirayu" last="Sa-Uth">Chirayu Sa-Uth</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, Faculty of Technology, Mahasarakham University, Maha Sarakham 44150, Thailand.</nlm:affiliation><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Biotechnology, Faculty of Technology, Mahasarakham University, Maha Sarakham 44150</wicri:regionArea><wicri:noRegion>Maha Sarakham 44150</wicri:noRegion></affiliation></author><author><name sortKey="Rattanasena, Paweena" sort="Rattanasena, Paweena" uniqKey="Rattanasena P" first="Paweena" last="Rattanasena">Paweena Rattanasena</name><affiliation wicri:level="1"><nlm:affiliation>Community Public Health Sub-Department, Department of Applied Sciences, Faculty of Science and Technology, Phranakhon Si Ayutthaya Rajabhat University, Ayutthaya 13000, Thailand.</nlm:affiliation><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Community Public Health Sub-Department, Department of Applied Sciences, Faculty of Science and Technology, Phranakhon Si Ayutthaya Rajabhat University, Ayutthaya 13000</wicri:regionArea><wicri:noRegion>Ayutthaya 13000</wicri:noRegion></affiliation></author><author><name sortKey="Chandrapatya, Angsumarn" sort="Chandrapatya, Angsumarn" uniqKey="Chandrapatya A" first="Angsumarn" last="Chandrapatya">Angsumarn Chandrapatya</name><affiliation wicri:level="1"><nlm:affiliation>Department of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.</nlm:affiliation><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok 10900</wicri:regionArea><wicri:noRegion>Bangkok 10900</wicri:noRegion></affiliation></author><author><name sortKey="Bussaman, Prapassorn" sort="Bussaman, Prapassorn" uniqKey="Bussaman P" first="Prapassorn" last="Bussaman">Prapassorn Bussaman</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, Faculty of Technology, Mahasarakham University, Maha Sarakham 44150, Thailand.</nlm:affiliation><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Biotechnology, Faculty of Technology, Mahasarakham University, Maha Sarakham 44150</wicri:regionArea><wicri:noRegion>Maha Sarakham 44150</wicri:noRegion></affiliation></author></analytic><series><title level="j">International journal of microbiology</title><idno type="ISSN">1687-918X</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><i>Xenorhabdus stockiae</i> PB09 bacterium has been shown to exhibit antifungal activity against several plant pathogens. To improve its efficacy, the optimization of the nutritional components in culture media was performed. The medium components that have significant effects on antifungal activity of <i>X. stockiae</i> PB09 were initially identified using a fractional factorial design. Response surface methodology and central composite design were then used to create a model for optimizing the levels of carbon, nitrogen, and mineral sources that maximize antifungal activity of <i>X. stockiae</i> PB09. After that, the suitable carbon, nitrogen, and mineral sources were selected and adjusted by the second-order polynomial regression model, which predicted that 98.62% of antifungal activity could be obtained when the medium contained sucrose, yeast extract, NaCl, and K<sub>2</sub>HPO<sub>4</sub> at 3.24, 23.71, 5.46, and 2.73 g/L, respectively. Laboratory verification of this recipe resulted in the antifungal activity at 97.95% in the shake flask experiment after 48-hour cultivation, which was significantly 27.22% higher than that obtained by using the TSB medium. In addition, <i>X. stockiae</i> PB09 cultured in the verified recipe by using 5 L fermenter could effectively inhibit the mycelial growth of <i>Phytophthora</i> sp., <i>Rhizoctonia solani</i>, <i>Pythium</i> sp., and <i>Fusarium oxysporum</i>. This study demonstrated that the RSM and CCD were shown to be valuable tools for optimizing the culture medium that maximize the antifungal activity of <i>X. stockiae</i> PB09.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">30008748</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>01</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1687-918X</ISSN><JournalIssue CitedMedium="Print"><Volume>2018</Volume><PubDate><Year>2018</Year></PubDate></JournalIssue><Title>International journal of microbiology</Title><ISOAbbreviation>Int J Microbiol</ISOAbbreviation></Journal><ArticleTitle>Modification of Medium Composition for Enhancing the Production of Antifungal Activity from <i>Xenorhabdus stockiae</i> PB09 by Using Response Surface Methodology.</ArticleTitle><Pagination><MedlinePgn>3965851</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1155/2018/3965851</ELocationID><Abstract><AbstractText><i>Xenorhabdus stockiae</i> PB09 bacterium has been shown to exhibit antifungal activity against several plant pathogens. To improve its efficacy, the optimization of the nutritional components in culture media was performed. The medium components that have significant effects on antifungal activity of <i>X. stockiae</i> PB09 were initially identified using a fractional factorial design. Response surface methodology and central composite design were then used to create a model for optimizing the levels of carbon, nitrogen, and mineral sources that maximize antifungal activity of <i>X. stockiae</i> PB09. After that, the suitable carbon, nitrogen, and mineral sources were selected and adjusted by the second-order polynomial regression model, which predicted that 98.62% of antifungal activity could be obtained when the medium contained sucrose, yeast extract, NaCl, and K<sub>2</sub>HPO<sub>4</sub> at 3.24, 23.71, 5.46, and 2.73 g/L, respectively. Laboratory verification of this recipe resulted in the antifungal activity at 97.95% in the shake flask experiment after 48-hour cultivation, which was significantly 27.22% higher than that obtained by using the TSB medium. In addition, <i>X. stockiae</i> PB09 cultured in the verified recipe by using 5 L fermenter could effectively inhibit the mycelial growth of <i>Phytophthora</i> sp., <i>Rhizoctonia solani</i>, <i>Pythium</i> sp., and <i>Fusarium oxysporum</i>. This study demonstrated that the RSM and CCD were shown to be valuable tools for optimizing the culture medium that maximize the antifungal activity of <i>X. stockiae</i> PB09.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Sa-Uth</LastName><ForeName>Chirayu</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, Faculty of Technology, Mahasarakham University, Maha Sarakham 44150, Thailand.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rattanasena</LastName><ForeName>Paweena</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Community Public Health Sub-Department, Department of Applied Sciences, Faculty of Science and Technology, Phranakhon Si Ayutthaya Rajabhat University, Ayutthaya 13000, Thailand.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chandrapatya</LastName><ForeName>Angsumarn</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bussaman</LastName><ForeName>Prapassorn</ForeName><Initials>P</Initials><Identifier Source="ORCID">0000-0002-5382-8130</Identifier><AffiliationInfo><Affiliation>Department of Biotechnology, Faculty of Technology, Mahasarakham University, Maha Sarakham 44150, Thailand.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>06</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>Egypt</Country><MedlineTA>Int J Microbiol</MedlineTA><NlmUniqueID>101516125</NlmUniqueID></MedlineJournalInfo></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>02</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>05</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>7</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>7</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>7</Month><Day>17</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30008748</ArticleId><ArticleId IdType="doi">10.1155/2018/3965851</ArticleId><ArticleId IdType="pmc">PMC6020484</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Microb Cell Fact. 2011 Nov 14;10:98</Citation><ArticleIdList><ArticleId IdType="pubmed">22082189</ArticleId></ArticleIdList></Reference><Reference><Citation>3 Biotech. 2016 Dec;6(2):139</Citation><ArticleIdList><ArticleId IdType="pubmed">28330211</ArticleId></ArticleIdList></Reference><Reference><Citation>J Nat Prod. 1995 Jul;58(7):1081-6</Citation><ArticleIdList><ArticleId IdType="pubmed">7561900</ArticleId></ArticleIdList></Reference><Reference><Citation>Microbiol Rev. 1996 Mar;60(1):21-43</Citation><ArticleIdList><ArticleId IdType="pubmed">8852894</ArticleId></ArticleIdList></Reference><Reference><Citation>J Invertebr Pathol. 2002 Mar;79(3):146-53</Citation><ArticleIdList><ArticleId IdType="pubmed">12133703</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Res Int. 2014;2014:293434</Citation><ArticleIdList><ArticleId IdType="pubmed">24864214</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Microbiol Biotechnol. 1990 Jan;32(4):449-54</Citation><ArticleIdList><ArticleId IdType="pubmed">1366394</ArticleId></ArticleIdList></Reference><Reference><Citation>J Invertebr Pathol. 2004 Jul;86(3):65-71</Citation><ArticleIdList><ArticleId IdType="pubmed">15261769</ArticleId></ArticleIdList></Reference><Reference><Citation>J Nat Prod. 1991 May-Jun;54(3):785-95</Citation><ArticleIdList><ArticleId IdType="pubmed">1955881</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioresour Technol. 2008 Apr;99(6):1708-15</Citation><ArticleIdList><ArticleId IdType="pubmed">17531470</ArticleId></ArticleIdList></Reference><Reference><Citation>J Appl Microbiol. 2009 Sep;107(3):746-59</Citation><ArticleIdList><ArticleId IdType="pubmed">19320949</ArticleId></ArticleIdList></Reference><Reference><Citation>Can J Microbiol. 1997 Aug;43(8):770-3</Citation><ArticleIdList><ArticleId IdType="pubmed">9304787</ArticleId></ArticleIdList></Reference><Reference><Citation>J Nat Prod. 1991 May-Jun;54(3):774-84</Citation><ArticleIdList><ArticleId IdType="pubmed">1955880</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioresour Technol. 2008 Nov;99(17):8245-51</Citation><ArticleIdList><ArticleId IdType="pubmed">18448333</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioresour Technol. 2010 Oct;101(19):7529-36</Citation><ArticleIdList><ArticleId IdType="pubmed">20488698</ArticleId></ArticleIdList></Reference><Reference><Citation>Int J Microbiol. 2013;2013:526260</Citation><ArticleIdList><ArticleId IdType="pubmed">24454383</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci Rep. 2014 Mar 06;4:4300</Citation><ArticleIdList><ArticleId IdType="pubmed">24599183</ArticleId></ArticleIdList></Reference><Reference><Citation>J Gen Microbiol. 1982 Dec;128(12):3061-5</Citation><ArticleIdList><ArticleId IdType="pubmed">7183749</ArticleId></ArticleIdList></Reference><Reference><Citation>J Zhejiang Univ Sci B. 2012 Apr;13(4):261-6</Citation><ArticleIdList><ArticleId IdType="pubmed">22467367</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Thaïlande</li></country></list><tree><country name="Thaïlande"><noRegion><name sortKey="Sa Uth, Chirayu" sort="Sa Uth, Chirayu" uniqKey="Sa Uth C" first="Chirayu" last="Sa-Uth">Chirayu Sa-Uth</name></noRegion><name sortKey="Bussaman, Prapassorn" sort="Bussaman, Prapassorn" uniqKey="Bussaman P" first="Prapassorn" last="Bussaman">Prapassorn Bussaman</name><name sortKey="Chandrapatya, Angsumarn" sort="Chandrapatya, Angsumarn" uniqKey="Chandrapatya A" first="Angsumarn" last="Chandrapatya">Angsumarn Chandrapatya</name><name sortKey="Rattanasena, Paweena" sort="Rattanasena, Paweena" uniqKey="Rattanasena P" first="Paweena" last="Rattanasena">Paweena Rattanasena</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhytophthoraV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000720 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000720 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PhytophthoraV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:30008748
|texte= Modification of Medium Composition for Enhancing the Production of Antifungal Activity from Xenorhabdus stockiae PB09 by Using Response Surface Methodology.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:30008748" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PhytophthoraV1
| This area was generated with Dilib version V0.6.38. |  |